Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface, while the MDDCs concentrated HIV to the same region. We propose that DC-T cell contact facilitates transmission of HIV by locally concentrating virus, receptor and coreceptor during the formation of an infectious synapse.